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Effect of single mismatches at 3′–end of primers on polymerase chain reaction
OBJECTIVE AND METHOD: To investigate the effect of three different mismatches (G/T, G/A or G/G) at the 3′–end of a primer to amplify a 268 bp (base pair) region of the human β–globin gene using different annealing temperatures (45 to 65°C). RESULTS: The primer with the G/T mismatch was as efficient...
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| Auteurs principaux: | , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
Sultan Qaboos University Medical Journal, College of Medicine & Health Sciences
2000
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| Sujets: | |
| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3174695/ https://ncbi.nlm.nih.gov/pubmed/24019700 |
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