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Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies.
We investigated the effects of various primer-template mismatches on DNA amplification of an HIV-1 gag region by the polymerase chain reaction (PCR). Single internal mismatches had no significant effect on PCR product yield while those at the 3'-terminal base had varied effects. A:G, G:A, and C...
Shranjeno v:
| izdano v: | Nucleic Acids Res |
|---|---|
| Main Authors: | , , , , , , |
| Format: | Artigo |
| Jezik: | Inglês |
| Izdano: |
Oxford University Press
1990
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| Online dostop: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330356/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2179874/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.4.999 |
| Oznake: |
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