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Quantitating primer-template interactions using deconstructed PCR

When the polymerase chain reaction (PCR) is used to amplify complex templates such as metagenomic DNA using single or degenerate primers, preferential amplification of templates (PCR bias) leads to a distorted representation of the original templates in the final amplicon pool. This bias can be infl...

Whakaahuatanga katoa

I tiakina i:
Ngā taipitopito rārangi puna kōrero
Ngā kaituhi matua: Jeremy Kahsen, Sonia K. Sherwani, Ankur Naqib, Trisha Jeon, Lok Yiu Ashley Wu, Stefan J. Green
Hōputu: Artigo
Reo:Inglês
I whakaputaina: PeerJ Inc. 2024-08-01
Rangatū:PeerJ
Ngā marau:
Urunga tuihono:https://peerj.com/articles/17787.pdf
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