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Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies.
We investigated the effects of various primer-template mismatches on DNA amplification of an HIV-1 gag region by the polymerase chain reaction (PCR). Single internal mismatches had no significant effect on PCR product yield while those at the 3'-terminal base had varied effects. A:G, G:A, and C...
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| Auteurs principaux: | , , , , , , |
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| Format: | Artigo |
| Langue: | Inglês |
| Publié: |
1990
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| Accès en ligne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC330356/ https://ncbi.nlm.nih.gov/pubmed/2179874 |
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