Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies.

We investigated the effects of various primer-template mismatches on DNA amplification of an HIV-1 gag region by the polymerase chain reaction (PCR). Single internal mismatches had no significant effect on PCR product yield while those at the 3'-terminal base had varied effects. A:G, G:A, and C...

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Hlavní autoři: Kwok, S, Kellogg, D E, McKinney, N, Spasic, D, Goda, L, Levenson, C, Sninsky, J J
Médium: Artigo
Jazyk:Inglês
Vydáno: 1990
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC330356/
https://ncbi.nlm.nih.gov/pubmed/2179874
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