Effects of primer-template mismatches on the polymerase chain reaction: human immunodeficiency virus type 1 model studies.

We investigated the effects of various primer-template mismatches on DNA amplification of an HIV-1 gag region by the polymerase chain reaction (PCR). Single internal mismatches had no significant effect on PCR product yield while those at the 3'-terminal base had varied effects. A:G, G:A, and C:C mi...

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Detalhes bibliográficos

Publicado no:	Nucleic Acids Res
Principais autores:	Kwok, S, Kellogg, D E, McKinney, N, Spasic, D, Goda, L, Levenson, C, Sninsky, J J
Formato:	Artigo
Idioma:	Inglês
Publicado em:	Oxford University Press 1990
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC330356/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2179874/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/18.4.999
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