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Selection for 3′-End Triplets for Polymerase Chain Reaction Primers
Primer extension by thermostable DNA polymerase in PCR starts from the 3′-end of a primer. If the PCR starting process fails, the entire PCR fails. Primer sequences at the 3′-end often interfere with success in PCR experiments. Over 2000 primer sequences from successful PCR experiments used with var...
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| Published in: | PCR Primer Design |
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| Main Author: | |
| Format: | Artigo |
| Language: | Inglês |
| Published: |
2007
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC7121268/ https://ncbi.nlm.nih.gov/pubmed/17951790 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-59745-528-2_3 |
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