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Selection for 3′ end triplets for polymerase chain reaction primers

The 3′ end of a primer is a key component of PCR primer design. Many recommendations for the composition and sequence of the 3′ end have been suggested based on theoretical considerations, but have not been verified experimentally. We analyzed 3′ end triplets of PCR primer sequences obtained from re...

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Publicado en:Mol Cell Probes
Autores principales: Onodera, Kenji, Melcher, Ulrich
Formato: Artigo
Lenguaje:Inglês
Publicado: Elsevier Ltd. 2004
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC7127618/
https://ncbi.nlm.nih.gov/pubmed/15488375
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mcp.2004.05.007
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