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Selection for 3′ end triplets for polymerase chain reaction primers

The 3′ end of a primer is a key component of PCR primer design. Many recommendations for the composition and sequence of the 3′ end have been suggested based on theoretical considerations, but have not been verified experimentally. We analyzed 3′ end triplets of PCR primer sequences obtained from re...

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Détails bibliographiques
Publié dans:Mol Cell Probes
Auteurs principaux: Onodera, Kenji, Melcher, Ulrich
Format: Artigo
Langue:Inglês
Publié: Elsevier Ltd. 2004
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC7127618/
https://ncbi.nlm.nih.gov/pubmed/15488375
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.mcp.2004.05.007
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