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Effect of single mismatches at 3′–end of primers on polymerase chain reaction

OBJECTIVE AND METHOD: To investigate the effect of three different mismatches (G/T, G/A or G/G) at the 3′–end of a primer to amplify a 268 bp (base pair) region of the human β–globin gene using different annealing temperatures (45 to 65°C). RESULTS: The primer with the G/T mismatch was as efficient...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: Simsek, M, Adnan, H
Format: Artigo
Sprache:Inglês
Veröffentlicht: Sultan Qaboos University Medical Journal, College of Medicine & Health Sciences 2000
Schlagworte:
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3174695/
https://ncbi.nlm.nih.gov/pubmed/24019700
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