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Deconstructing the Polymerase Chain Reaction: Understanding and Correcting Bias Associated with Primer Degeneracies and Primer-Template Mismatches

The polymerase chain reaction (PCR) is sensitive to mismatches between primer and template, and mismatches can lead to inefficient amplification of targeted regions of DNA template. In PCRs in which a degenerate primer pool is employed, each primer can behave differently. Therefore, inefficiencies d...

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書目詳細資料
發表在:PLoS One
Main Authors: Green, Stefan J., Venkatramanan, Raghavee, Naqib, Ankur
格式: Artigo
語言:Inglês
出版: Public Library of Science 2015
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在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC4440812/
https://ncbi.nlm.nih.gov/pubmed/25996930
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0128122
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