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Deconstructing the Polymerase Chain Reaction II: an improved workflow and effects on artifact formation and primer degeneracy
Polymerase chain reaction (PCR) amplification of complex microbial genomic DNA templates with degenerate primers can lead to distortion of the underlying community structure due to inefficient primer-template interactions leading to bias. We previously described a method of deconstructed PCR (“PEX P...
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| Publicado no: | PeerJ |
|---|---|
| Main Authors: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
PeerJ Inc.
2019
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC6573857/ https://ncbi.nlm.nih.gov/pubmed/31231597 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.7717/peerj.7121 |
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