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Importance of primer selection for the detection of hepatitis C virus RNA with the polymerase chain reaction assay.

We compared four primer sets from conserved regions of the hepatitis C virus (HCV) genome for their ability to detect HCV RNA in a "nested" cDNA polymerase chain reaction assay on sera from 114 anti-HCV antibody-positive individuals from around the world. The different primer sets had equi...

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Detalhes bibliográficos
Main Authors: Bukh, J, Purcell, R H, Miller, R H
Formato: Artigo
Idioma:Inglês
Publicado em: 1992
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC48201/
https://ncbi.nlm.nih.gov/pubmed/1309604
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