Importance of primer selection for the detection of hepatitis C virus RNA with the polymerase chain reaction assay.

We compared four primer sets from conserved regions of the hepatitis C virus (HCV) genome for their ability to detect HCV RNA in a "nested" cDNA polymerase chain reaction assay on sera from 114 anti-HCV antibody-positive individuals from around the world. The different primer sets had equivalent sen...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Bukh, J, Purcell, R H, Miller, R H
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1992
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC48201/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1309604/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.89.1.187
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