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Small-Molecule Hydrophobic Tagging Induced Degradation of HaloTag Fusion Proteins

The ability to regulate any protein of interest in living systems with small molecules remains a challenge. We hypothesized that appending a hydrophobic moiety to the surface of a protein would mimic the partially denatured state of the protein, thus engaging the cellular quality control machinery t...

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Autors principals: Neklesa, Taavi K., Tae, Hyun Seop, Schneekloth, Ashley R., Stulberg, Michael J., Corson, Timothy W., Sundberg, Thomas B., Raina, Kanak, Holley, Scott A., Crews, Craig M.
Format: Artigo
Idioma:Inglês
Publicat: 2011
Matèries:
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3139752/
https://ncbi.nlm.nih.gov/pubmed/21725302
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nchembio.597
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