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The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing
BACKGROUND: The use of RNAi to analyse gene function in vitro is now widely applied in biological research. However, several difficulties are associated with its use in vivo, mainly relating to inefficient delivery and non-specific effects of short RNA duplexes in animal models. The latter can lead...
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| 主要な著者: | , , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
BioMed Central
2011
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3123217/ https://ncbi.nlm.nih.gov/pubmed/21612654 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-4-148 |
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