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Quantification of siRNA using competitive qPCR

We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the huma...

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Bibliografski detalji
Glavni autori: Liu, Wei-li, Stevenson, Mark, Seymour, Leonard W., Fisher, Kerry D.
Format: Artigo
Jezik:Inglês
Izdano: Oxford University Press 2009
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2615599/
https://ncbi.nlm.nih.gov/pubmed/19004870
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn903
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