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Quantification of siRNA using competitive qPCR
We have developed a PCR-based short interfering RNA (siRNA) quantification method based on competition between siRNA and a homologous DNA primer for annealing to template DNA, avoiding the requirement for prior conversion of RNA to cDNA. Primers and probe were designed to amplify regions of the huma...
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| Main Authors: | , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Oxford University Press
2009
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2615599/ https://ncbi.nlm.nih.gov/pubmed/19004870 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gkn903 |
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