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Detection of siRNA induced mRNA silencing by RT-qPCR: considerations for experimental design
BACKGROUND: RNA interference (RNAi) has been one of the most rapidly expanding areas of biological research in the past decade, revolutionizing the ability to analyze gene function. Thorough validation of siRNA duplexes is required prior to use in experimental systems, ideally by western blotting to...
में बचाया:
| मुख्य लेखकों: | , , , |
|---|---|
| स्वरूप: | Artigo |
| भाषा: | Inglês |
| प्रकाशित: |
BioMed Central
2010
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| विषय: | |
| ऑनलाइन पहुंच: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2850347/ https://ncbi.nlm.nih.gov/pubmed/20199660 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-3-53 |
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