Detection of siRNA induced mRNA silencing by RT-qPCR: considerations for experimental design

BACKGROUND: RNA interference (RNAi) has been one of the most rapidly expanding areas of biological research in the past decade, revolutionizing the ability to analyze gene function. Thorough validation of siRNA duplexes is required prior to use in experimental systems, ideally by western blotting to...

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Main Authors: Holmes, Katherine, Williams, Catrin M, Chapman, Elinor A, Cross, Michael J
Format: Artigo
Jezik:Inglês
Izdano: BioMed Central 2010
Teme:
Short Report
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2850347/
https://ncbi.nlm.nih.gov/pubmed/20199660
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-3-53
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