The importance of RT-qPCR primer design for the detection of siRNA-mediated mRNA silencing

BACKGROUND: The use of RNAi to analyse gene function in vitro is now widely applied in biological research. However, several difficulties are associated with its use in vivo, mainly relating to inefficient delivery and non-specific effects of short RNA duplexes in animal models. The latter can lead...

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Bibliographic Details
Main Authors: Herbert, Mike, Coppieters, Natacha, Lasham, Annette, Cao, Helen, Reid, Glen
Format: Artigo
Language:Inglês
Published: BioMed Central 2011
Subjects:
Short Report
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3123217/
https://ncbi.nlm.nih.gov/pubmed/21612654
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-4-148
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