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Design and synthesis of polyacrylamide-based oligonucleotide supports for use in nucleic acid diagnostics.
Polyacrylamide supports, in a range of pore sizes, were investigated as nucleic acid affinity matrices for the detection of target DNA or RNA sequences using a sandwich hybridization format. Bromoacetyl and thiol oligonucleotide derivatives were covalently linked to sulfhydryl- and bromoacetyl-polya...
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| Main Authors: | , , , |
|---|---|
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
1993
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| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC309420/ https://ncbi.nlm.nih.gov/pubmed/7684127 |
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