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Reversible suppression of an essential gene in adult mice using transgenic RNA interference

RNAi has revolutionized loss-of-function genetics by enabling sequence-specific suppression of virtually any gene. Furthermore, tetracycline response elements (TRE) can drive expression of short hairpin RNAs (shRNAs) for inducible and reversible target gene suppression. Here, we demonstrate the feas...

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Detalhes bibliográficos
Main Authors: McJunkin, Katherine, Mazurek, Anthony, Premsrirut, Prem K., Zuber, Johannes, Dow, Lukas E., Simon, Janelle, Stillman, Bruce, Lowe, Scott W.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3084121/
https://ncbi.nlm.nih.gov/pubmed/21482754
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1104097108
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