Efficient targeted gene disruption in the soma and germ line of the frog Xenopus tropicalis using engineered zinc-finger nucleases

The frog Xenopus, an important research organism in cell and developmental biology, currently lacks tools for targeted mutagenesis. Here, we address this problem by genome editing with zinc-finger nucleases (ZFNs). ZFNs directed against an eGFP transgene in Xenopus tropicalis induced mutations consi...

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Bibliographic Details
Main Authors: Young, John J., Cherone, Jennifer M., Doyon, Yannick, Ankoudinova, Irina, Faraji, Farhoud M., Lee, Andrew H., Ngo, Catherine, Guschin, Dmitry Y., Paschon, David E., Miller, Jeffrey C., Zhang, Lei, Rebar, Edward J., Gregory, Philip D., Urnov, Fyodor D., Harland, Richard M., Zeitler, Bryan
Format: Artigo
Language:Inglês
Published: National Academy of Sciences 2011
Subjects:
Biological Sciences
Online Access:https://ncbi.nlm.nih.gov/pmc/articles/PMC3084115/
https://ncbi.nlm.nih.gov/pubmed/21471457
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1102030108
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