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Direct selection of cDNAs using whole chromosomes.
We have developed a method for direct selection of cDNAs using whole chromosomes as target DNA. Double-strand cDNAs were synthesized from human fetal brain polyadenylated mRNAs. Flow-sorted chromosomes 17 and 19 were amplified by degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR)...
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| Main Authors: | , , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
1995
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| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC307398/ https://ncbi.nlm.nih.gov/pubmed/7501464 |
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