Size-selection of cDNA libraries for the cloning of cDNAs after suppression subtractive hybridization
Here we describe the establishment of size-selected cDNA libraries for the cloning of full-length cDNAs that were initially identified by suppression subtractive hybridization (SSH) technology as being differentially expressed. First, the SSH-cDNA fragments were used as 32P-probes to verify their le...
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| Principais autores: | , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
Taylor & Francis Group
2003-07-01
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| Series: | BioTechniques |
| Acceso en liña: | https://www.future-science.com/doi/10.2144/03351st02 |
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