Direct selection of cDNAs using whole chromosomes.

We have developed a method for direct selection of cDNAs using whole chromosomes as target DNA. Double-strand cDNAs were synthesized from human fetal brain polyadenylated mRNAs. Flow-sorted chromosomes 17 and 19 were amplified by degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR)...

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Autors principals: Rouquier, S, Trask, B J, Taviaux, S, van den Engh, G, Diriong, S, Lennon, G G, Giorgi, D
Format: Artigo
Idioma:Inglês
Publicat: 1995
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC307398/
https://ncbi.nlm.nih.gov/pubmed/7501464
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