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Direct selection of cDNAs using whole chromosomes.

We have developed a method for direct selection of cDNAs using whole chromosomes as target DNA. Double-strand cDNAs were synthesized from human fetal brain polyadenylated mRNAs. Flow-sorted chromosomes 17 and 19 were amplified by degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR)...

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Detalles Bibliográficos
Publicado en:Nucleic Acids Res
Main Authors: Rouquier, S, Trask, B J, Taviaux, S, van den Engh, G, Diriong, S, Lennon, G G, Giorgi, D
Formato: Artigo
Idioma:Inglês
Publicado: Oxford University Press 1995
Acceso en liña:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307398/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7501464/
https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.21.4415
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