Direct selection of cDNAs using whole chromosomes.

We have developed a method for direct selection of cDNAs using whole chromosomes as target DNA. Double-strand cDNAs were synthesized from human fetal brain polyadenylated mRNAs. Flow-sorted chromosomes 17 and 19 were amplified by degenerate oligonucleotide primed polymerase chain reaction (DOP-PCR)...

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Bibliografiske detaljer

Udgivet i:	Nucleic Acids Res
Principais autores:	Rouquier, S, Trask, B J, Taviaux, S, van den Engh, G, Diriong, S, Lennon, G G, Giorgi, D
Format:	Artigo
Sprog:	Inglês
Udgivet:	Oxford University Press 1995
Online adgang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC307398/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/7501464/ https://ncbi.nlm.nih.govhttps://doi.org/10.1093/nar/23.21.4415
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