Construction of a uniform-abundance (normalized) cDNA library.
We have used a kinetic approach to construct cDNA libraries containing approximately equal representations of all sequences in a preparation of poly(A)+ RNA. Randomly primed cDNA fragments of a selected size range were cloned in lambda phage vector. Inserts were amplified by the polymerase chain rea...
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| Publicado no: | Proc Natl Acad Sci U S A |
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| Principais autores: | , , |
| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
National Academy of Sciences
1991
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC51142/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1705712/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.5.1943 |
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