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Construction of a uniform-abundance (normalized) cDNA library.

We have used a kinetic approach to construct cDNA libraries containing approximately equal representations of all sequences in a preparation of poly(A)+ RNA. Randomly primed cDNA fragments of a selected size range were cloned in lambda phage vector. Inserts were amplified by the polymerase chain rea...

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Hlavní autoři: Patanjali, S R, Parimoo, S, Weissman, S M
Médium: Artigo
Jazyk:Inglês
Vydáno: 1991
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC51142/
https://ncbi.nlm.nih.gov/pubmed/1705712
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