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cDNA selection: efficient PCR approach for the selection of cDNAs encoded in large chromosomal DNA fragments.

Identification of coding segments in large fragments of genomic DNA is a recurrent problem in genome mapping and positional cloning studies. We have developed a rapid and efficient protocol to achieve this goal, based on hybridization of cDNA fragments to immobilized DNA and recovery of the selected...

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Hlavní autoři: Parimoo, S, Patanjali, S R, Shukla, H, Chaplin, D D, Weissman, S M
Médium: Artigo
Jazyk:Inglês
Vydáno: 1991
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC52770/
https://ncbi.nlm.nih.gov/pubmed/1946377
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