cDNA selection: efficient PCR approach for the selection of cDNAs encoded in large chromosomal DNA fragments.

Identification of coding segments in large fragments of genomic DNA is a recurrent problem in genome mapping and positional cloning studies. We have developed a rapid and efficient protocol to achieve this goal, based on hybridization of cDNA fragments to immobilized DNA and recovery of the selected...

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Detalhes bibliográficos

Publicado no:	Proc Natl Acad Sci U S A
Principais autores:	Parimoo, S, Patanjali, S R, Shukla, H, Chaplin, D D, Weissman, S M
Formato:	Artigo
Idioma:	Inglês
Publicado em:	National Academy of Sciences 1991
Assuntos:	Research Article
Acesso em linha:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC52770/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/1946377/ https://ncbi.nlm.nih.govhttps://doi.org/10.1073/pnas.88.21.9623
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