Construction and characterization of a normalized cDNA library.

We have developed a simple procedure based on reassociation kinetics that can reduce effectively the high variation in abundance among the clones of a cDNA library that represent individual mRNA species. For this normalization, we used as a model system a library of human infant brain cDNAs that wer...

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Autors principals: Soares, M B, Bonaldo, M F, Jelene, P, Su, L, Lawton, L, Efstratiadis, A
Format: Artigo
Idioma:Inglês
Publicat: 1994
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC44785/
https://ncbi.nlm.nih.gov/pubmed/7937745
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