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Construction and characterization of a normalized cDNA library.

We have developed a simple procedure based on reassociation kinetics that can reduce effectively the high variation in abundance among the clones of a cDNA library that represent individual mRNA species. For this normalization, we used as a model system a library of human infant brain cDNAs that wer...

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Detalhes bibliográficos
Main Authors: Soares, M B, Bonaldo, M F, Jelene, P, Su, L, Lawton, L, Efstratiadis, A
Formato: Artigo
Idioma:Inglês
Publicado em: 1994
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC44785/
https://ncbi.nlm.nih.gov/pubmed/7937745
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