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cDNA selection: efficient PCR approach for the selection of cDNAs encoded in large chromosomal DNA fragments.
Identification of coding segments in large fragments of genomic DNA is a recurrent problem in genome mapping and positional cloning studies. We have developed a rapid and efficient protocol to achieve this goal, based on hybridization of cDNA fragments to immobilized DNA and recovery of the selected...
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Main Authors: | , , , , |
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格式: | Artigo |
語言: | Inglês |
出版: |
1991
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主題: | |
在線閱讀: | https://ncbi.nlm.nih.gov/pmc/articles/PMC52770/ https://ncbi.nlm.nih.gov/pubmed/1946377 |
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