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Simultaneous single-molecule measurements of phage T7 replisome composition and function reveal the mechanism of polymerase exchange

A complete understanding of the molecular mechanisms underlying the functioning of large, multiprotein complexes requires experimental tools capable of simultaneously visualizing molecular architecture and enzymatic activity in real time. We developed a novel single-molecule assay that combines the...

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Detalhes bibliográficos
Main Authors: Loparo, Joseph J., Kulczyk, Arkadiusz W., Richardson, Charles C., van Oijen, Antoine M.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2011
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3048139/
https://ncbi.nlm.nih.gov/pubmed/21245349
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1018824108
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