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Direct Evidence for Conformational Heterogeneity in Human Pancreatic Glucokinase from High-Resolution NMR

High-resolution NMR is used to investigate the conformational dynamics of human glucokinase, a 52 kDa monomeric enzyme that displays kinetic cooperativity. (1)H-(15)N TROSY spectra of uniformly labeled glucokinase, collected in the absence and presence of glucose, reveal significant cross-peak overl...

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Bibliografische gegevens
Hoofdauteurs: Larion, Mioara, Salinas, Roberto K., Bruschweiler-Li, Lei, Brüschweiler, Rafael, Miller, Brian G.
Formaat: Artigo
Taal:Inglês
Gepubliceerd in: 2010
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Online toegang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3023983/
https://ncbi.nlm.nih.gov/pubmed/20735087
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi101098f
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