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Direct Evidence for Conformational Heterogeneity in Human Pancreatic Glucokinase from High-Resolution NMR
High-resolution NMR is used to investigate the conformational dynamics of human glucokinase, a 52 kDa monomeric enzyme that displays kinetic cooperativity. (1)H-(15)N TROSY spectra of uniformly labeled glucokinase, collected in the absence and presence of glucose, reveal significant cross-peak overl...
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| Main Authors: | , , , , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
2010
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3023983/ https://ncbi.nlm.nih.gov/pubmed/20735087 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi101098f |
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