Complementation of a Saccharomyces cerevisiae ERG11/CYP51 (Sterol 14α-Demethylase) Doxycycline-Regulated Mutant and Screening of the Azole Sensitivity of Aspergillus fumigatus Isoenzymes CYP51A and CYP51B

Aspergillus fumigatus sterol 14α-demethylase isoenzymes CYP51A and CYP51B were heterologously expressed in a Saccharomyces cerevisiae mutant (YUG37-erg11), wherein native ERG11/CYP51 expression is controlled using a doxycycline-regulatable promoter. When cultured in the presence of doxycycline, reco...

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Main Authors: Martel, Claire M., Parker, Josie E., Warrilow, Andrew G. S., Rolley, Nicola J., Kelly, Steven L., Kelly, Diane E.
格式: Artigo
語言:Inglês
出版: American Society for Microbiology (ASM) 2010
主題:
Susceptibility
在線閱讀:https://ncbi.nlm.nih.gov/pmc/articles/PMC2976139/
https://ncbi.nlm.nih.gov/pubmed/20733045
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AAC.00349-10
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