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The Dynamics of DNA Topoisomerase IIα in Living Cells
Here we describe methods to prepare a mammalian expression plasmid encoding EGFP fused to the amino terminus of human DNA topoisomerase IIα (TopoIIα). This plasmid is transfected into LLC-Pk cells, a porcine epithelial cell line that remains relatively flat during mitosis. After selection for stable...
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| Main Authors: | , , |
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| Formáid: | Artigo |
| Teanga: | Inglês |
| Foilsithe: |
2009
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| Ábhair: | |
| Rochtain Ar Líne: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2950002/ https://ncbi.nlm.nih.gov/pubmed/19763954 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60761-340-4_18 |
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