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The Dynamics of DNA Topoisomerase IIα in Living Cells

Here we describe methods to prepare a mammalian expression plasmid encoding EGFP fused to the amino terminus of human DNA topoisomerase IIα (TopoIIα). This plasmid is transfected into LLC-Pk cells, a porcine epithelial cell line that remains relatively flat during mitosis. After selection for stable...

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Detalhes bibliográficos
Main Authors: Daum, John R., Mo, Yin Yuan, Gorbsky, Gary J.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2950002/
https://ncbi.nlm.nih.gov/pubmed/19763954
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1007/978-1-60761-340-4_18
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