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Combinatorial Enzyme Design Probes Allostery and Cooperativity in the Trypsin Fold
Converting one enzyme into another is challenging due to the uneven distribution of important amino acids for function in both protein sequence and structure. We report a strategy for protein engineering allowing an organized mixing and matching of genetic material that leverages lower throughput wi...
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| Main Authors: | , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2908009/ https://ncbi.nlm.nih.gov/pubmed/20399789 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2010.04.024 |
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