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Engineering protein allostery: 1.05 Å resolution structure and enzymatic properties of a Na(+)-activated trypsin
Some trypsin-like proteases are endowed with Na(+)-dependent allosteric enhancement of catalytic activity, but this important mechanism has been difficult to engineer in other members of the family. Replacement of nineteen amino acids in Streptomyces griseus trypsin targeting the active site and the...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2008
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2423008/ https://ncbi.nlm.nih.gov/pubmed/18377928 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jmb.2008.03.003 |
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