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Mutagenesis and Evolution of Sulfated Antibodies using an Expanded Genetic Code

To facilitate the biochemical study of posttranslationally modified proteins, we have developed a strategy in which otherwise posttranslationally modified amino acids are genetically encoded in E. coli in response to unique nonsense or frameshift codons. Here, we illustrate the utility of this appro...

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Detalhes bibliográficos
Main Authors: Liu, Chang C., Choe, Hyeryun, Farzan, Michael, Smider, Vaughn V., Schultz, Peter G.
Formato: Artigo
Idioma:Inglês
Publicado em: 2009
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2891052/
https://ncbi.nlm.nih.gov/pubmed/19715291
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi9011429
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