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Mutagenesis and Evolution of Sulfated Antibodies using an Expanded Genetic Code
To facilitate the biochemical study of posttranslationally modified proteins, we have developed a strategy in which otherwise posttranslationally modified amino acids are genetically encoded in E. coli in response to unique nonsense or frameshift codons. Here, we illustrate the utility of this appro...
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Main Authors: | , , , , |
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Formato: | Artigo |
Idioma: | Inglês |
Publicado em: |
2009
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Assuntos: | |
Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2891052/ https://ncbi.nlm.nih.gov/pubmed/19715291 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi9011429 |
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