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Efficient expression of tyrosine-sulfated proteins in E. coli using an expanded genetic code
Tyrosine sulfation is an important posttranslational modification that occurs in higher eukaryotes and is involved in cell-cell communication, viral entry, and adhesion. We describe a protocol for the heterologous expression of selectively tyrosine-sulfated proteins in E. coli through the use of an...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2928070/ https://ncbi.nlm.nih.gov/pubmed/20010929 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.188 |
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