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Efficient expression of tyrosine-sulfated proteins in E. coli using an expanded genetic code
Tyrosine sulfation is an important posttranslational modification that occurs in higher eukaryotes and is involved in cell-cell communication, viral entry, and adhesion. We describe a protocol for the heterologous expression of selectively tyrosine-sulfated proteins in E. coli through the use of an...
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| 主要な著者: | , , , |
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| フォーマット: | Artigo |
| 言語: | Inglês |
| 出版事項: |
2009
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| 主題: | |
| オンライン･アクセス: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2928070/ https://ncbi.nlm.nih.gov/pubmed/20010929 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.188 |
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