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Efficient expression of tyrosine-sulfated proteins in E. coli using an expanded genetic code

Tyrosine sulfation is an important posttranslational modification that occurs in higher eukaryotes and is involved in cell-cell communication, viral entry, and adhesion. We describe a protocol for the heterologous expression of selectively tyrosine-sulfated proteins in E. coli through the use of an...

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Bibliografski detalji
Glavni autori: Liu, Chang C., Cellitti, Susan E., Geierstanger, Bernhard H., Schultz, Peter G.
Format: Artigo
Jezik:Inglês
Izdano: 2009
Teme:
Online pristup:https://ncbi.nlm.nih.gov/pmc/articles/PMC2928070/
https://ncbi.nlm.nih.gov/pubmed/20010929
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nprot.2009.188
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