Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs

A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific polymerase chai...

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Bibliografske podrobnosti
Main Authors: Fang, Huafeng, Shen, Yuefei, Taylor, John-Stephen
Format: Artigo
Jezik:Inglês
Izdano: Cold Spring Harbor Laboratory Press 2010
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Method
Online dostop:https://ncbi.nlm.nih.gov/pmc/articles/PMC2885691/
https://ncbi.nlm.nih.gov/pubmed/20498459
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1940610
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