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Native mRNA antisense-accessible sites library for the selection of antisense oligonucleotides, PNAs, and siRNAs
A procedure for rapidly generating a library of antisense-accessible sites on native mRNAs (mRNA antisense-accessible sites library [MASL]) is described that involves reverse transcription of whole cell mRNA extracts with a random oligodeoxynucleotide primer followed by mRNA-specific polymerase chai...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Cold Spring Harbor Laboratory Press
2010
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2885691/ https://ncbi.nlm.nih.gov/pubmed/20498459 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1261/rna.1940610 |
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