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Histidine Residue Mediates Radical-induced Hinge Cleavage of Human IgG1

Hydroxyl radicals induce hinge cleavage in a human IgG1 molecule via initial radical formation at the first hinge Cys(231) followed by electron transfer to the upper hinge residues. To enable engineering of a stable monoclonal antibody hinge, we investigated the role of the hinge His(229) residue us...

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Autores principales: Yates, Zac, Gunasekaran, Kannan, Zhou, Hongxing, Hu, Zhonghua, Liu, Zhi, Ketchem, Randal R., Yan, Boxu
Formato: Artigo
Lenguaje:Inglês
Publicado: American Society for Biochemistry and Molecular Biology 2010
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Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC2881791/
https://ncbi.nlm.nih.gov/pubmed/20304919
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M110.108597
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